Antigen retrieval is necessary when you perform the IHC experiments on FFPE tissue. The most common Antigen retrieval method is Heat Induced Epitope Retrieval (HIER). And there are three important factors to consider for successful HIER:
- Optimal heating time
- Good preparation of tissue section
- Specific type of antigen retrieval solution
Optimal heating time: The heating time has to be optimized for each antigen and appliance.
Good preparation of tissue section: OriGene has more than 140,000 human tissue samples from U.S. medical centers using the industry’s most strict ethical requirements and IRB protocols. For the complete list of our tissue section, please check the following OriGene website: http://www.origene.com/Tissue/TissueSearch.aspx
Specific type of antigen retrieval solution: The main difference among different retrieval solutions is pH. Citrate solution is acidic, while EDTA solution is basic. Some antibodies may request an enzymatic antigen retrieval solution, like Trpsin and Pepsin solution (RTU) from OriGene (SKU# GB300016 and GB300017). You will have to optimize your solution to get the best staining result. OriGene has all the solutions that you need for your IHC. You can find the complete product list from the following link.